KPL's new ProteoSOLTM Tissue Extraction System enables the relative quantitative analysis of protein expression from formalin-fixed, paraffin-embedded (FFPE) tissue. The system is based on the Liquid Tissue® process, coupling the strength of histology to enable visualization of normal and pathologic tissue morphology with the ease of dot blot detection. It provides a simple protocol for tissue procurement and protein extraction from formalin-fixed tissue samples and for spotting protein samples on membranes. Samples can be arrayed and detected using high-throughput detection methods. Immunodetection with specific antibodies allows the direct comparison of a specific protein across multiple samples in a single array.
KPL’s Protein DetectorTM Microarray Kits will soon be available and provide optimized reagents for either alkaline phosphatase colorimetric or chemiluminescent detection. They can be used with a variety of protein arrays, including arrays created from ProteoSOL tissue extracts.