DyLight 800 Absorption and Emission Spectra
DyLight 800-labeled antibody conjugates produce a far infrared color that is not visible to the naked eye. DyLight 800 dye is excited at 770 nm and has an emission peak at 794 nm where autofluorescence is low. DyLight 800 conjugates remain intensely fluorescent over a broad pH range (pH 4-9). They compare favorably with IRDye 800 conjugates in all critical parameters, and serve as a reliable replacement in a variety of detection applications, such as fluorescence microscopy, Western blotting, In-Cell Westerns, On-Cell Westerns, tissue section imaging and array platforms.
||See our line of DyLight 680-labeled conjugates.
DyLight 800 Dye
Spectrally similar to IRDye 800 by LI-COR, the DyLight 800 dye has a fluorescence excitation maximum at 770 nm and an emission maximum at 794 nm.
Multicolor Infrared Fluorescence Detection in Blotting Applications
Recent developments in Western blotting involve using near-infrared fluorescence as an imaging platform. DyLight 680 and 800 conjugates provide sensitive, quantitative analysis over a wide dynamic range in blotting applications with these outstanding benefits:
- Secondary antibodies with defined specificity and sensitivity
- Brighter signal than visible fluorescence
- Quantitation accuracy superior to traditional methods
- Broader dynamic range than chemiluminescence
- Virtually no background autofluorescence for most biological specimens
- Easy to assay multiple proteins simultaneously on one Western blot
- Time to results significantly faster than chemiluminescence techniques
- Cost-effective - eliminates need for chemiluminescence substrates, film and darkroom
- Clean results - no bleeding from consecutive lanes
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