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Protein Detector Western Blot Kits
Membrane-based detection assays offer versatile and powerful methods for characterization and analysis or proteins. Immunoblotting can take many forms, including the commonly known Western blotting and dot blotting applications. In Western blotting techniques, antigens are immobilized on a membrane following electrophoretic separation on a gel. Dot blotting allows the antigen of interest to be directly attached to the membrane from a protein solution. See our Procedural Guide for more tips on successful blotting.
KPL offers numerous products, including Protein Detector Western Blotting Systems, for membrane- based detection of proteins. The choice of specific protocols for membrane based immunodetection will depend on a number of factors, including the degree of sensitivity, the purity of the antigen, and the reagents and equipment available to the researcher. KPL's stand alone reagents and complete kits provide convenience and flexibility for optimizing these factors and therefore serve as an excellent choice for the researcher detecting proteins in membrane based applications. |
When analyzing proteins immobilized on membrane, Protein Detector Western Blotting Kits provide consistent, high quality results with a selection of chemiluminescent and chromogenic systems to meet a variety of research needs. These comprehensive kits allow the detection and quantification of specific antigens in a complex protein mixture. Combining proprietary and easy-to-use substrates, a unique blocking agent and highly specific secondary antibodies in optimized protocols, Protein Detector Western Blotting Kits offer the highest signal-to-noise ratio available. They enable clean, clear results, blot after blot.
Chromogenic and Chemiluminescent Substrates for Western Blotting
The final step in an enzyme immunoassay is the addition of substrate that allows visualization and quantification of the sample. Upon reaction with enzyme conjugate, substrate is converted to either a colored or luminescent end product proportional to the amount of target analyte in the sample. Chromogenic substrates produce color which can be monitored visually for rapid qualitative results, or with the use of a densitometer for quantitative measurement. Chemiluminescent substrates produce light, which can be measured using a luminometer or permanently recorded through exposure to X-ray film.
KPL offers an extensive line of stable, liquid substrates optimized for membrane-based assays. See our Overview of Blotting Substrates for colorimetric and chemiluminescent membrane detection. The range of substrates available from KPL enables detection over a wide range of analyte concentrations. KPL blotting substrates are designed as convenient systems for ease of use. They provide a high degree of assay to assay precision, maximum stability and sensitivity.
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