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Overview of Chemiluminescent Blotting Substrates
The following table summarizes the features of KPL's chemiluminescent peroxidase and phosphatase substrates
to help you compare and choose the best substrate for your application.
A table comparing KPL's colorimetric blotting substrates can be viewed here.
|
Substrate |
PhosphaGLO |
PhosphaGLO Reserve |
LumiGLO Reserve |
LumiGLO |
|
Type |
Chemiluminescent |
Chemiluminescent |
Chemiluminescent |
Chemiluminescent |
|
Enzyme System |
AP |
AP |
HRP |
HRP |
|
Format |
1-C |
1-C |
2-C |
2-C |
|
Detection Limit |
Picogram |
Femtogram |
Sub-picogram; femtogram |
0.3 pg nucleic acid
6.0 pg protein
|
|
Detection Method |
Film/Chemi-luminescent imager |
Film/Chemi-luminescent imager |
Film or Image Analysis |
Film or Image Analysis |
|
Stability of Working Solution |
2 years at 4o C |
2 years at 4o C |
8 hours |
24 hours |
|
Kinetics |
15 minutes |
15 minutes |
5 minutes |
5 minutes |
|
Recommended Membrane |
Nitrocellulose or PVDF |
Nitrocellulose or PVDF |
Nitrocellulose or PVDF |
Nitrocellulose, nylon, or PVDF |
C=Component
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