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Overview of Colorimetric Blotting Substrates
The following table summarizes the features of KPL's peroxidase and phosphatase colorimetric substrates
to help you compare and choose the best substrate for your application.
A table comparing KPL's chemiluminescent blotting substrates can be viewed here.
|
Substrate |
TMB 1-C |
TMB 3-C |
4 CN |
FirePhos |
BCIP/NBT |
BCIP/NBT |
|
Type |
Chromogenic |
Chromogenic |
Chromogenic |
Chromogenic |
Chromogenic |
Chromogenic |
|
Enzyme System |
HRP |
HRP |
HRP |
AP |
AP |
AP |
|
Format |
1-C |
3-C |
2-C |
1-C |
1-C |
3-C |
|
Detection Limit |
50 pg protein |
50 pg protein |
500 pg protein |
Low ng |
500 pg-1 ng |
500 pg-1 ng |
|
Detection Method |
Visual |
Visual |
Visual |
Visual |
Visual |
Visual |
|
Stability of Working Solution |
NA |
24 hours |
1 hour |
NA |
NA |
1 hour |
|
Kinetics |
10 minutes |
10 minutes |
10 minutes |
10 minutes |
10 minutes |
10 minutes |
|
Recommended Membrane |
Nitrocellulose or PVDF |
Nitrocellulose or PVDF |
Nitrocellulose or PVDF |
Nitrocellulose or PVDF |
Nylon or PVDF |
Nylon or PVDF |
C=Component NA = Not Applicable
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