- Used with pNPP tablets to create a working phosphatase substrate
- Stabilizes pNPP in solution
DEA buffer is used to dissolve pNPP tablets and prepare a working solution of pNPP Microwell Substrate. The working substrate develops a soluble yellow product and is used to detect alkaline-phosphatase conjugates. The substrate reaction can be stopped with an equal volume of APstop Solution.
Detection Method: ELISA
Formulation: 5M Diethanolamine (5X)
Measurement: 405 - 410 nm
Stability: 1 year
Storage: 2 - 8oC