-Maximum sensitivity; Detection of less than 0.4 ng/mL of HRP.

-Less than 10% lot-to-lot variation.

-Water-based substrate; contains no DMF or DMSO.

KPL's TMB substrates (SureBlue Reserve, SureBlue and TMB) produce high O.D. values with SureBlue Reserve exhibiting the highest sensitivity.  SureBlue and TMB demonstrate lower O.D. values and are comparable in performance.  ABTS 1-  and 2-component yield lower O.D. values than TMB while exhibiting similar performance.

SureBlue Reserve^TM provides maximum sensitivity for detection of peroxidase labeled conjugates in a microwell format and provides 50% more sensitivity than standard SureBlue TMB substrate. The substrate develops a deep blue color that is measurable at 650 nm and turns yellow when stopped by acidification and read at 450 nm. Single component, ready to use format of 3,3', 5, 5'-Tetramethylbenzidine. No reagent preparation required, very convenient. Stable for 2 years from data of manufacture when stored at 4^oC.