Product Highlights

- Most sensitive colorimetric AP substrate for blotting

- Sharp band resolution and minimal background

- Reaction is linear over a wide dynamic range.

BCIP/NBT Phosphatase Substrate (1-Component) detects proteins that have been immobilized on membranes following Western blot transfer or directly via dot blotting. The system is based on the hydrolysis of 5-bromo, 4-chloro, 3-indolylphosphate (BCIP) and nitroblue tetrazolium (NBT) by alkaline phosphatase, resulting in a strong purple reaction product and sensitivity in the low nanogram range. The color is stable and resists fading when exposed to light. 

Detection Method: Colorimetric blotting

Formulation: 1-component, ready-to-use solution

Measurement: Direct visualization

Stability: 1 year

Storage: 2 - 8^oC