Products > Western Blotting Kits and Reagents > Colorimetric Blotting Substrates

BCIP/NBT Phosphatase Substrate (1-Component)
1 x 100 mL BCIP/NBT Phosphatase Substrate (1-Component)
Cat. #: 50-81-18
Size: 100 mL
List Price: $49.60

Prices valid for U.S. customers only.
International customers please contact your local distributor or
to place an order.

Product Highlights

- Most sensitive colorimetric AP substrate for blotting

- Sharp band resolution and minimal background

- Reaction is linear over a wide dynamic range.

BCIP/NBT Phosphatase Substrate detects proteins that have been immobilized on membranes following Western blot transfer or directly via dot blotting. The system is based on the hydrolysis of 5-bromo, 4-chloro, 3-indolylphosphate (BCIP) and nitroblue tetrazolium (NBT) by alkaline phosphatase, resulting in a strong purple reaction product and sensitivity in the low nanogram range. The color is stable and resists fading when exposed to light. 


Detection Method: Colorimetric Western or dot blotting

Stability: 1 year from date of receipt

Storage: 2 - 8oC


A Comparison of Blocking Solutions Used in Western Blotting

BCIP and NBT Storage in High-Density Polyethylene Bottles

Comparison of BCIP/NBT Phosphatase Substrates: One vs. Three Component Systems

Phosphatase Membrane Colorimetric Substrates

Select Your Optimal Detection Method

Stability of One Component BCIP/NBT Substrate

Western Blotting Brochure

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