Products > Western Blotting Kits and Reagents > Chemiluminescent Blotting Substrates

LumiGLO Chemiluminescent Substrate
1 X 120 mL LumiGLO Chemiluminescent Substrate A
1 X 120 mL LumiGLO Chemiluminescent Substrate B
Cat. #: 54-61-00
Size: 240 mL
List Price: $154.90

Prices valid for U.S. customers only.
International customers please contact your local distributor or
to place an order.

Product Highlights

- Reliable and economical; ideal for routine blotting applications

- Superior signal-to-noise ratio

- Produces a sustained, linear signal for multiple exposures on film

LumiGLO® Chemiluminescent Substrate detects proteins that have been immobilized on membranes following Western blot transfer or directly via dot blotting. LumiGLO is a luminol-based substrate that enables picogram-level detection of peroxidase conjugates. After reaction with HRP conjugate, light emission reaches maximum intensity within 5 minutes and is sustained for approximately 1 - 2 hours.


Detection Method: Chemiluminescent Western, Southern, and dot blotting, ELISA

Stability: 1 year from date of receipt

Storage: 2 - 8oC


A Comparison of Blocking Solutions Used in Western Blotting

Comparison of LumiGLO® Chemiluminescent Peroxidase Substrate System to Brand 2

ELISA Brochure

KPL Chemi Substrates and Syngene's Image Analyser Enable Sensitive Western Detection

Optimization of Assay Conditions Using LumiGLO Reserve vs. LumiGLO Chemiluminescent Substrates in Western Blotting

Quick Guide - Five Fast Fixes for WB Disasters

Select Your Optimal Detection Method

Stability of LumiGLO® Chemiluminescent Peroxidase Substrate System

Time Course for LumiGLO HRP Chemiluminescent Substrates on Nitrocellulose Membrane Application Note

Western Blotting Brochure

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