- High specificity to E. coli O121 with minimal cross-reactivity to other E. coli serogroups
- Affinity purified polyclonal antibody yields higher sensitivity than monoclonal antibodies
- Antibody conjugated with long carbon spacer arm biotin - minimizing steric hindrance between biotinylated antibody and streptavidin
Affinity purified polyclonal antibody to E. coli O121 made in goat is conjugated to biotin. Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serogroup O121. Certain strains of the E. coli serogroup O121 are considered to be STECs (shiga-toxin producing E. coli). Each lot is tested to assure specificity and lot-to-lot consistency using KPL’s in-house ELISA assay.
Immunogen: Heat-killed whole cells of E. coli serotype O121
Purification: Affinity purified
Specificity: E. coli O121
Form: Whole IgG
Physical State: Lyophilized
Stabilizers/Preservatives: Goat serum is added as a protein stabilizer. No preservative added. Additional biological protection may be provided with 0.1% sodium azide.
Suggested Working Dilutions: ELISA 0.5-0.1 µg/mL, Blotting 0.5-0.1 μg/mL, Histo/Cytochemical Processes 10.0-1.0 μg/mL
Stability: 1 year from date of receipt
Storage: 2 - 8°C
The plot depicts OD values from a TMB-detected HRP capture ELISA. KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O121 antibody (KPL catalog no. 01-95-95) was used as the capture antibody, and HRP-labeled conjugate (KPL catalog no. 04-95-95) was used as the reporter. All other assay components were KPL catalog products.
BacTrace Anti-E.coli O121 antibody is also available unlabeled and labeled with peroxidase and DyLight 488.