BacTrace® Anti-Escherichia coli O121 Antibody, Biotin-labeled
1 x 0.5 mg biotin-labeled antibody to Escherichia coli O121
Cat. #: 16-95-95
Size: 0.5 mg
List Price: $286.00

Prices valid for U.S. customers only.
International customers please contact your local distributor or kplcustserv@seracare.com
to place an order.

Product Highlights
  • High specificity to E. coli O121 with minimal cross-reactivity to other E. coli serogroups
  • Affinity purified polyclonal antibody yields higher sensitivity than monoclonal antibodies
  • Antibody conjugated with long carbon spacer arm biotin - minimizing steric hindrance between biotinylated antibody and streptavidin

Affinity purified polyclonal antibody to E. coli O121 made in goat is conjugated to biotin.  Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serogroup O121. Certain strains of the E. coli serogroup O121 are considered to be STECs (shiga-toxin producing E. coli). Each lot is tested to assure specificity and lot-to-lot consistency using KPL’s in-house ELISA assay.

 

Specifications

Immunogen: Heat-killed whole cells of E. coli serotype O121

Purification: Affinity purified

Specificity: E. coli O121

Host: Goat

Form: Whole IgG

Label: Biotin

Physical State: Lyophilized

Stabilizers/Preservatives: Goat serum is added as a protein stabilizer. No preservative added. Additional biological protection may be provided with 0.1% sodium azide.

Suggested Working Dilutions: ELISA 0.5-0.1 µg/mL, Blotting 0.5-0.1 μg/mL, Histo/Cytochemical Processes 10.0-1.0 μg/mL

Stability: 1 year from date of receipt

Storage: 2 - 8°C

 

 

The plot depicts OD values from a TMB-detected HRP capture ELISA. KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O121 antibody (KPL catalog no. 01-95-95) was used as the capture antibody, and HRP-labeled conjugate (KPL catalog no. 04-95-95) was used as the reporter. All other assay components were KPL catalog products.

BacTrace Anti-E.coli O121 antibody is also available unlabeled and labeled with peroxidase and DyLight 488.

Quantity:


Documents
MSDS
Datasheet
BacTrace Non-O157 STEC Antibodies Flyer

Rapid Detection of STEC Strains Using Label-free Biosensor


Related Products
Cat. # Product Name Size
01-95-95 BacTrace® Anti-Escherichia coli O121 Antibody 1.0 mg
011-95-95 BacTrace® Anti-Escherichia coli O121 Antibody 0.1 mg
04-95-95 BacTrace® Anti-Escherichia coli O121 Antibody, Peroxidase-labeled 0.1 mg
072-03-95-95 BacTrace® Anti-Escherichia coli O121 Antibody, DyLight 488-labeled 0.5 mg
50-95-95 BacTrace® Escherichia coli O121:H19 Positive Control 1 vial