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BacTrace® Anti-Escherichia coli O157:H7 Antibody, Biotin-labeled
1 x 0.5 mg vial BacTrace Anti-Escherichia coli O157:H7 Antibody, Biotin-labeled
Cat. #: 16-95-90
Size: 0.5 mg
List Price: $224.00

Prices valid for U.S. customers only.
International customers please contact your local distributor or kplcustserv@seracare.com
to place an order.

Product Highlights

  • High sensitivity - polyclonal antibody recognizes multiple epitopes vs. single-epitope recognition of monoclonals
  • High specificity - provides serotype-specific detection of E. coli O157:H7
  • Lot-to-lot consistency

BacTrace® Anti-E. coli O157:H7 Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serotype O157. Biotin-labeled antibodies are recommended for use with KPL's labeled streptavidin for increased sensitivity in immunoassays.

Specifications

Immunogen: Heat-killed whole cells of E. coli serotype O157:H7

Purification: Affinity purification

Specificity: E. coli O157:H7

Host: Goat

Form: Whole IgG

Label: Biotin with a long carbon spacer arm

Physical State: Lyophilized

Stabilizers/Preservatives: Goat serum added as protein stabilizer

Suggested Working Dilutions: Microwell assays: 1:1000 (0.5 µg/mL) - 1:5000 (0.1 µg/mL), Membrane assays: 1:1000 (0.5 µg/mL) - 1:5000 (0.1 µg/mL), Immunohistology: 1:50 - 1:500 (10 µg/mL - 1.0 µg/mL) 

Stability: 1 year from date of receipt

Storage: 2 - 8°C

 

            Specificity of BacTrace Anti-E.coli Antibody

Specificity of E. coli

Colorimetric ELISA showing reactivity of Anti-E.coli O157:H7 Antibody with biotin/peroxidase-streptavidin. 

 

View larger version

 

 

 

The image below demonstrates the high specificity of BacTrace Anti-E.coli O157 for shiga toxin (stx)-producing E. coli O157:H7 with virtually no cross-reactivity to other stx-producing E.coli or non-STEC bacteria.  

The plot depicts OD values from a TMB detected HRP capture-ELISA. A 1:100 dilution (~107 CFU/mL) of KPL heat-killed positive controls from various STEC serogroups and S. Typhimurium was used as analytes. Unlabeled Anti-E. coli O157:H7 antibody (KPL catalog # 01-95-90) was used as the capture antibodies and HRP-labeled cognate conjugates (KPL catalog # 04-95-90) were used as reporters. All other assay components were KPL catalog products, following manufacturer’s protocol.
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