- High specificity to E. coli O26, minimal cross-reactivity to other E. coli serogroups
- Polyclonal antibody yields higher sensitivity than monoclonal antibodies
- Standardized lots provide consistent performance
BacTrace® Antibody to E. coli O26 is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serogroup O26. Certain strains of the E. coli serogroup O26 are considered to be STECs (shiga toxin-producing E. coli). Biotin-labeled antibodies are recommended for use with KPL's labeled streptavidin for increased sensitivity in immunoassays.
Immunogen: Heat-killed whole cells of E. coli serotype O26
Purification: Affinity purification
Specificity: E. coli O26
Form: Whole IgG
Label: Biotin with long carbon spacer arm
Physical State: Lyophilized
Stabilizers/Preservatives: Goat serum added as protein stabilizer
Suggested Working Dilutions: FLISA: 1:100 – 1:1,000 (10.0 µg/mL - 1.0 µg/mL) Blotting: 1:7500 – 1:15,000 (0.13 µg/mL - 0.067 µg/mL), Immunohistology: 1:100 – 1:1,000 (10.0 µg/mL - 1.0 µg/mL)
Stability: 1 year from date of receipt
Storage: 2 - 8°C
The plot depicts OD values from a TMB-detected HRP capture-ELISA. KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O26 antibody (KPL catalog no. 01-95-92) was used as the capture antibody and HRP-labeled conjugate (KPL catalog no. 04-95-92) was used as the reporter. All other assay components were KPL catalog products. This study demonstrates the high specificity of BacTrace Anti-E. coli O26 in an ELISA. Cross reactivity is minimal with other E. coli groups and non-E. coli bacteria.