- High specificity to E. coli O26, minimal cross-reactivity to other E. coli serogroups
- Polyclonal antibody yields higher sensitivity than monoclonal antibodies
- Standardized lots provide consistent performance
BacTrace® Anti-E. coli O26 Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E.coli serogroup O26. Certain strains of the E. coli serogroup O26 are considered to be STECs (shiga toxin-producing E. coli). This antibody can be used in a variety of immunoselection and immunodetection applications.
Immunogen: Heat-killed whole cells of E. coli serotype O26
Purification: Affinity purification
Specificity: E. coli O26
Form: Whole IgG
Physical State: Lyophilized
Suggested Working Dilutions: Capture Antibody: 10 μg/mL, Primary Antibody: 1 – 2 μg/mL
Stability: 1 year from date of receipt
Storage: 2 - 8°C
The plot depicts OD values from a TMB-detected HRP capture ELISA. KPL heat-killed postive controls from various STEC serogroups and S. Typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O26 antibody (KPL catalog # 01-95-92) was used as the capture antibody and HRP-labeled cognate conjugate (KPL catalog # 04-95-92) were used as reporter. All other assay components were KPL catalog products, following manufacturer’s protocol. This study demonstrates the high specificity of BacTrace Anti-E.coli O26 in an ELISA.