- High specificity to E. coli O26, minimal cross-reactivity to other E. coli serogroups
- Polyclonal antibody yields higher sensitivity than monoclonal antibodies
- Standardized lots provide consistent performance
BacTrace® Anti-Escherichia coli O26 Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serogroup O26. This antibody demonstrates minimal cross-reactivity to other E. coli serogroups. Certain strains of the E. coli serogroup O26 are considered to be STECs (shiga toxin-producing E. coli). The antibody is conjugated with DyLight 488, a brighter, more photostable alternative to FITC and Cy dyes. It can be used in a variety of immunoselection and immunodetection applications.
Immunogen: Heat-killed whole cells of E. coli serotype O26
Purification: Affinity purification
Specificity: E. coli O26
Form: Whole IgG
Label: DyLight 488 molar fluorophore/antibody protein ratio: 5:1 - 10:1
Excitation/Emission Wavelength: 493/518 nm
Physical State: Lyophilized
Stabilizers/Preservatives: Bovine serum albumin is added as protein stabilizer. 0.02% sodium azide added as a preservative.
Suggested Working Dilutions: FLISA: 1:100 – 1:1,000 (10.0 µg/mL - 1.0 µg/mL), Blotting: 1:7500 – 1:15,000 (0.13 µg/mL - 0.067 µg/mL), Immunohistology: 1:100 – 1:1,000 (10.0 µg/mL - 1.0 µg/mL)
Stability: 1 year from date of receipt
Storage: 2 - 8°C
The plot depicts OD values from a TMB-detected HRP capture ELISA. KPL heat-killed positive controls from various STEC serogroups and S. typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O26 antibody (KPL catalog no. 01-95-92) was used as the capture antibody and HRP-labeled conjugate (KPL catalog # 04-95-92) was used as the reporter. All other assay components were KPL catalog products. This study demonstrates the high specificity of BacTrace Anti-E.coli O26 in an ELISA.