- High specificity to E. coli O26, minimal cross-reactivity to other E. coli serogroups
- Polyclonal antibody yields higher sensitivity than monoclonal antibodies
- Standardized lots provide consistent performance
BacTrace® Anti-E. coli O26 Antibody is isolated from a serum pool of goats immunized with heat-killed whole cells of E.coli serogroup O26. Certain strains of the E. coli serogroup O26 are considered to be STECs (shiga toxin-producing E. coli). This antibody can be used in a variety of immunoselection and immunodetection applications.
Immunogen: Heat-killed whole cells of E. coli serotype O26
Purification: Affinity purification
Specificity: E. coli O26
Form: Whole IgG
Label: Horseradish peroxidase
Molar enzyme/antibody protein ratio: 4:1
Physical State: Lyophilized
Stabilizers/Preservatives: Goat serum added as protein stabilizer
Suggested Working Dilutions: ELISA: 1:1000 - 1:5000 (0.1 µg/mL - 0.02 µg/mL), Blotting: (0.1 µg/mL - 0.02 µg/mL), Immunohistology:1:50 - 1:500 2.0 µg/mL - 0.2 µg/mL
Stability: 1 year from date of receipt
Storage: 2 - 8°C
The plot depicts OD values from a TMB-detected HRP capture ELISA. KPL heat-killed positive controls from various STEC serogroups and S. Typhimurium (1:100 dilution, ~107 CFU/mL) were used as analytes. Unlabeled Anti-E. coli O26 antibody (KPL catalog no. 01-95-92) was used as the capture antibody and HRP-labeled conjugate (KPL catalog # 04-95-92) was used as reporter. All other assay components were KPL catalog products. This study demonstrates the high specificity of BacTrace Anti-E. coli O26 in an ELISA.